Nuclear Receptor 4a3 (Nr4a3) Regulates Murine Mast Cell Responses and Granule Content
Figure 2
Nr4a3 affects cytokine/chemokine secretion in response to FcεeRI cross-linking.
(A–D) WT and Nr4a3−/− mast cells were incubated with TNP-specific IgE over-night followed by activation for 4 hours or 24 hours with TNP-OVA as indicated. Supernatants were analyzed for levels of MCP-1 (A), TNFα (B), IL-6 (C) and IL-13 (D) by ELISA (T-test, n = 4; *p≤0.05). The lack of Nr4a3 is associated with a reduction in cytokine release following FcεRI cross-linking.