Dissecting the Molecular Mechanism of Ionizing Radiation-Induced Tissue Damage in the Feather Follicle
Figure 5
Involvement of Stat1 gene in IR-induced feather defects.
(A) RT-PCR and densitometrical quantification showing increased Stat1 expression in the feather follicles. **, p<0.01; ***, p<0.001. (B) Stat1 antibody staining showing nuclear enrichment after IR exposure (T1 samples). (C) Whole-mount view of the feather epithelium after lentiviral-mediated Stat1 over-expression in the feather follicle. In control experiments, a lentivirus carrying GFP was used. (D) H&E analysis of Stat1 over-expressed feather follicle showing abnormal epithelial branching. Samples were collected 2 weeks after virus infection. Bar = 100 µm.