High-Dose Irradiation Induces Cell Cycle Arrest, Apoptosis, and Developmental Defects during Drosophila Oogenesis
Figure 2
IR-induced S-phase checkpoint in mitotically dividing germline cells in wild type and checkpoint mutant females.
The BrdU incorporation assay was performed in wild type and checkpoint mutant ovaries 1(A) Representative images of a wild type germarium assayed for BrdU incorporation (green) in the germline cells before and after irradiation are shown. The DNA was stained with DAPI (blue). Bar, 10 µm. (B) The number of germariums containing a strong BrdU signal in region 1 were counted. The values represent the relative percentage of germarium containing BrdU-positive germline cells in irradiated samples compared to the untreated controls. The data are presented as the means and standard deviations of three independent experiments. The total number of germarium for each cell type at different time points were at least 220. * p = 0.0016 (the grp mutant versus the grp mnk double mutant).