Anti-Osteoclastogenic Activity of Praeruptorin A via Inhibition of p38/Akt-c-Fos-NFATc1 Signaling and PLCγ-Independent Ca2+ Oscillation
Figure 5
Effect of praeruptorin A on RANKL-induced Ca2+ oscillation and PLCγ phosphorylation.
(A) The effect of praeruptorin A on the RANKL-induced Ca2+ oscillation was evaluated as described in ‘Materials and Methods’. Each trace presents intracellular Ca2+ mobilization in each cell. (B) The effect of praeruptorin A on the RANKL-induced phosphorylation of PLCγ was evaluated by Western blot analysis. BMMs were pre-treated with praeruptorin A for 2 h before treatment with RANKL. Actin was used as an internal control. Densitometric analysis was performed using ImageJ software and the relative, normalized ratio of p-PLCγ2/PLCγ2 was presented.