A Palindromic CpG-Containing Phosphodiester Oligodeoxynucleotide as a Mucosal Adjuvant Stimulates Plasmacytoid Dendritic Cell-Mediated TH1 Immunity
Figure 5
G9.1-induced TH1-type Ab production was completely pDC-dependent.
Anti-mPDCA-1-treated and isotype control-treated BALB/c mice (n = 4) were administered DT (2 Lf) plus G9.1 (20 µg) nasally twice at a 1-month interval. Serum titers of anti-DT IgG1 and IgG2a were measured as described in Fig. 3. The percentage of pDCs in splenocytes decreased from 0.18 to 0.01 after anti-mPDCA-1 treatment (lower panel). The pDC-depleted mice exhibited no G9.1-induced IgG2a production (upper panel). *p<0.05 and **p<0.01 as determined by t-test. Three independent experiments yielded nearly identical results.