A Single Sfp-Type Phosphopantetheinyl Transferase Plays a Major Role in the Biosynthesis of PKS and NRPS Derived Metabolites in Streptomyces ambofaciens ATCC23877
Figure 5
Effect of the deletion of sco6673-like on the spiramycin and congocidine production.
(A) Spiramycin and congocidine production was assessed directly by HPLC from supernatant samples collected from the culture of S. ambofaciens ATCC23877 wild-type and mutant strains grown in liquid MP5. Commercial spiramycin (100 µl at 0.1 mg/ml) was used as control. Absorption was monitored at 232 nm. The inserts are an enlargement of the area between 20 to 35 min of retention time containing the peaks corresponding to the spiramycin (spiramycin is a mixture of three forms). The peak corresponding to congocidine is highlighted with a black dot. (B) UV spectra (from 200 to 350 nm) of the peaks highlighted with an asterisk and corresponding to spiramycin.