Treatment with N- and C-Terminal Peptides of Parathyroid Hormone-Related Protein Partly Compensate the Skeletal Abnormalities in IGF-I Deficient Mice
Figure 2
Bone structure in wild type and Igf1-null mice with or without PTHrP treatment.
(A) Representative images of femur size (left) and the growth plate of the tibia stained with Masson’s trichrome (right) from wild type (WT) and Igf1-null mice. Growth plate width, and trabecular number in a defined area below the growth plate, were measured and represented for each genotype. Statistical differences were analyzed by unpaired Student’s t test. Values are expressed as mean ± SEM corresponding to 2 males and 2 females for each genotype. **p<0.01 vs WT mice. (B) Representative µCT images from trabecular and cortical regions of the distal femur from WT and Igf1-null mice, treated or not with PTHrP (1–36) (Nt) or osteostatin (Ost). Images were adjusted to the same appearance for easy comparison between WT and Igf1-null mice which have smaller bones. Thus, scale bars represent 1 and 0.5 mm for trabecular bone (upper panel) or 200 and 100 µm for cortical bone (lower panel) from WT and Igf1-null mice, respectively. The regions of interest in trabecular and cortical segments of the mouse bone which were analyzed by µCT are depicted at the top.