ICAM-1-Based Rabies Virus Vaccine Shows Increased Infection and Activation of Primary Murine B Cells In Vitro and Enhanced Antibody Titers In-Vivo
Figure 3
rRABV infection results in the upregulation of ICAM-1 expression, which is augmented by infection with rRABV-mICAM-1.
Total naïve primary murine splenocytes were infected as described in Figure 2 and then stained for B220, ICAM-1, and RABV-N for analysis by flow cytometry. A) Representative gating strategy of total live lymphocytes gated on ICAM-1 and RABV N staining. B) Representative gating strategy of B220+ B cells gated on RABV N and ICAM-1. C) Percent ICAM-1+ cells in the total live lymphocyte population. D) Percent ICAM-1+ cells in the B220+ cell population. E) Percent RABV N+ ICAM-1+ cells in the total live lymphocyte population. D) Percent RABV N+ ICAM-1+ cells in the B220+ cell population. To compare two groups of data, we used an unpaired, two-tailed Student's t test. (* p<0.05; **, p<0.01; ***, p<0.001)