Regulation of Ethanol-Related Behavior and Ethanol Metabolism by the Corazonin Neurons and Corazonin Receptor in Drosophila melanogaster
Figure 2
Generation of CrzR-null allele.
(A) Diagram of CrzR encoding exons and approximate locations of PCR primers. Exons and introns are shown as arrows and solid lines, respectively. Coding exons are shown in grey, and UTRs in white. An 8-kb deletion was indicated by the hatched box. A footprint of gal4 coding region and flanking sequence derived from the Minos element was found at the original insertion site. (B) PCR products derived from the designated primer sets and mutant genomic DNA. No specific PCR product was produced from f3 primer. (C) RT-PCR. CrzR01 did not produce PCR product, while the wild-type did it with expected size.