The Monocyte to Macrophage Transition in the Murine Sterile Wound
Figure 3
Migration of blood monocyte subsets to the wound.
Ly6Chi and Ly6Clow blood monocytes were selectively labeled with fluorescent microparticles (MP) following treatment with or without liposome-encapsulated clodronate (clo-lip) as described in Materials & Methods. C57BL/6J mice were then wounded for 1 or 7 days and accumulation of labeled monocytes/macrophages in the wound was assessed by flow cytometry. (A) Schematic of clo-lip and MP administration for the selective labeling of Ly6Chi monocytes. Mice were wounded 1 day after MP delivery. (B) F4/80+ blood and wound cells were examined by flow cytometry following clodronate treatment and MP labeling. In blood and the day 1 and day 7 wound, Ly6Chi monocytes were predominantly labeled with MPs. (C) Schematic of MP treatment for the selective labeling of circulating Ly6Clow monocytes prior to wounding for 1 or 7 days. (D) F4/80+ blood and wound cells were examined by flow cytometry following selective labeling of Ly6Clow monocytes. MP-labeled Ly6Clow monocytes were identified in the circulation but not in the day 1 or day 7 wound. The proportion of cells in quadrants is indicated by numbers on plots. N = 2 mice per group.