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Protein Kinase C Phosphomimetics Alter Thin Filament Ca²⁺ Binding Properties

Figure 2

Effect of phosphorylation mimicking protein modifications on the rate of Ca²⁺ dissociation from the Tn complex.

Panel A shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , TnI T144E , TnI T144D and TnI T144A . Panel B shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , TnI S43/45E , TnI S43/45D , TnI S43/45A , and TnI S43/45E/T144E . Panel C shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , TnT T194E , S198E , T284E and TnT T203E . The data traces have been staggered and normalized for clarity.

doi: https://doi.org/10.1371/journal.pone.0086279.g002