Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
Figure 5
Bim is a direct target of miR-214 in NPC cell lines.
(A). Pairing of miR-214 with Bim 3′UTR region and BAX 3′UTR region. (B and C). qRT-PCR and Western blot showed that knockdown of miR-214 using LNA-antimiR-214 led to upregulation of endogenous Bim and BAX in both CNE2 and SUNE1 cells, respectively. (D) Overexpression of miR-214 by the miR-214 mimic resulted in a significant decrease in luciferase signals of pMIRreport- Bim 3′UTR transfected 293T cells, but not in pMIRreport- BAX 3′UTR transfected 293T cells.