The Maternal ITPK1 Gene Polymorphism Is Associated with Neural Tube Defects in a High-Risk Chinese Population
Figure 2
The Comparison of Wt-Oligos and Mut-Oligos in the ability of transcription factors combination.
Line 1, the blank control without the nuclear extracts from HeLa cell; Line 2, the Biotin Wt-probe were incubated with the nuclear extracts from HeLa cell; Line 3–4, competition was also performed using Cold Wt Oligos and Cold Mt Oligos; Line 5, the negative control of supershift line, incubated with IgG; Line 6–7, performed with antibodies specific for c-fos, c-jun; Line 8–10, the Mut-probe were showed for the control. These results showed a different allelic binding capacity of AP-1 in rs3783903 of the ITPK1, which is affected by an A→G exchange.