Determining the Relative Contribution and Hierarchy of hha and qseBC in the Regulation of Flagellar Motility of Escherichia coli O157:H7
Figure 4
Transcriptional activation of qseC by Hha.
The effect of hha on the transcription of qseC was determined by a QRT-PCR assay using a DNA-free RNA prepared from bacterial strains grown in DMEM for 5 h to an A600 of 1.1 to 1.2. The relative expression of qseC in the parent, hha (Δhha), and hha-complemented (hha+/Δhha) mutant strains is shown in the bar graph as means ± SEM of three independent bacterial cultures and nine technical replicates. Significant (p<0.05) differences in qseC transcription between the parent and mutant strains were determined using Mann-Whitney test. ** (p = 0.0019); **** (p = <0.0001).