Proper PIN1 Distribution Is Needed for Root Negative Phototropism in Arabidopsis
Figure 3
Subcellular localization of PIN1 in the roots of plants grown in the dark or exposed to blue light.
(A–B) Co-localization (yellow) of PIN1-GFP (green) with ER or Golgi tracker dye (red) in the dark. Four-day-old etiolated seedlings of the PIN1::PIN1-GFP (green) marker line were pretreated with either ER tracker dye (A) or Golgi tracker dye (B) for 30 minutes in the dark before imaging. (C) Co-localization of PIN1-GFP (green) with FM 4–64 (red) after 2 h of blue light illumination. Four-day-old etiolated seedlings of the PIN1::PIN1-GFP marker line were exposed to blue light (10 µmol m−2sec−1) for 2 h, and treated with FM 4–64 for 10 minutes before imaging. (A–C) At least twelve seedlings were imaged per line for each of three replicates. Bars = 10 µm. Panels in the left are enlargements of the boxed regions shown in the rightmost column.