Reprogramming to Pluripotency through a Somatic Stem Cell Intermediate
Figure 2
iNdiPSCs exhibit a pluripotent global gene expression profile.
(A) Heat map of microarray data showing similar gene expression patterns of iNdiPSC-1 and -2 (both passage 10) compared with ESCs, NSC 2F iPSCs (passage 7, 2F: Oct4 and Klf4), and MEF 4F iPSCs (passage 7, 4F: Oct4, Sox2, Klf4, and Myc). MEFs, NSCs, and iNSCs serve as controls. Color bar at the top indicates gene expression in log2 scale. Red and blue colors represent high and low expression levels, respectively. (B) Hierarchical clustering and (C) principal component analysis (PCA) of the different cell types depicted in (A). (D) – (F) Pairwise scatter plots comparing the gene expression profile of iNdiPSCs-1 and -2 with MEFs (D), iNSCs (E), and ESCs (F). Specifically highlighted are the pluripotency marker genes Oct4, Sox2, Lin28, and Klf4. Black lines indicate boundaries of 2-fold difference in gene expression levels. Scattering density is indicated in the bar on the right: the higher the density, the darker the color. Gene expression levels are depicted in log2 scale. The number of differentially expressed genes (No of diff.) is listed below each plot.