Dickkopf-1 Is Oncogenic and Involved in Invasive Growth in Non Small Cell Lung Cancer
Figure 3
Effects of DKK1 overexpression in 95C cell.
(A) RT-PCR and Western blot analysis of the DKK1 expression. DKK1 level in 95C cell transfected with pCMV-Tag-2b-DKK1 is significantly higher than that in pCMV-Tag-2b and blank control group. (B) The wounded and healing 95C cells. (C) Measurement of migration distance (*P<0.05). (D) The invasion ability of 95C transfected with pCMV-Tag-2b-DKK1 was significantly enhanced. (E) The number of cells migrating through the Matrigel-coated filters was statistic analyzed. Assays were done in triplicate wells (*P<0.01).