Thermogenic Ability of Uncoupling Protein 1 in Beige Adipocytes in Mice
Figure 1
Effect of cold acclimation on cell morphology and uncoupling protein 1 expression in adipose tissues.
Mice were housed in a cold environment (10°C; cold-acclimated mice) or a control environment (24°C; control mice) for 3 weeks. (A) Sections of interscapular brown adipose tissue (BAT), inguinal white adipose tissue (WAT) (I-WAT), and perigonadal WAT (G-WAT) were stained with hematoxylin and eosin (HE) or immunostained using antibody against uncoupling protein 1 (UCP1). (B) UCP1 protein expression was analyzed by western blotting. To detect UCP1, 2.5 µg (BAT) or 20 µg (I-WAT and G-WAT) of protein was used. Values are means ± SE for 4 mice. *p<0.05, versus the control group.