Salinomycin Activates AMP-Activated Protein Kinase-Dependent Autophagy in Cultured Osteoblastoma Cells: A Negative Regulator against Cell Apoptosis
Figure 2
Autophagy inhibition enhances salinomycin-induced cytotoxicity in osteoblastoma cells.
U2OS cells were treated with indicated concentration of salinomycin (Sali, 0.1, 1, 5 and 10 μM) for 48 hours (A), or treated with 10 μM of salinomycin for indicated time (B), cell viability was analyzed by CCK-8 cell viability assay. U2OS and MG-63 cells were treated with vehicle (0.1 % of DMSO) or salinomycin (Sali, 10 μM) in the presence or abscess of 3-MA (0.5 or 1 mM) for 48 hours, cell viability was analyzed by CCK-8 assay (C and D). Scramble RNAi- or LC3B siRNA-transfected HEK-293T cells were treated with salinomycin (Sali, 5 and 10 μM), cell viability was analyzed (E), and LC3B and tubulin expression was also examined to confirm the transfection efficiency (F). Experiments in this figure were repeated three times. #p<0.05 vs. vehicle (“V”) group. *p<0.05.