Effect of Glycyrrhizin on Pseudomonal Skin Infections in Human-Mouse Chimeras
Figure 2
HBD-1 production in patient chimeras treated with glycyrrhizin.
A. Patient chimeras created with burn patient #1∼#3 unburned skin tissues and their CD31+ IMC were treated i.p. with glycyrrhizin (10 mg/kg) 6 and 24 hours after the IMC inoculation. As a control, the chimeras were treated with saline (0.2 ml/mouse). Twenty-four hours after the final glycyrrhizin treatment, 5 skin biopsies were obtained from grafted site tissues, homogenized together, and assayed for HBD-1 by ELISA. A white bar shows the results obtained from a group of mice grafted with skin alone. Fig. 2A-1 shows 3 independent experiments performed using skin and blood specimens from 3 patients, and Fig. 2A-2 shows mean ± SEM of the results shown in Fig. 2A-1. **P<0.01 vs saline-treated control. B. The recovery of HBD-1 production in patient chimeras treated with glycyrrhizin. The chimeras created with unburned skin tissues from patients #4∼#7 and their CD31+ IMC were treated twice with 1 to 10 mg/kg of glycyrrhizin. As controls, the chimeras were treated with saline (0.2 ml/mouse). Twenty-four hours after the treatment, 5 skin biopsies were obtained from grafted site tissues, homogenized together, and assayed for HBD-1 by ELISA. A white bar shows the results obtained from a group of mice grafted with skin alone (mean ± SEM of the 4 independent experiments). *P<0.05; **P<0.01 vs saline-treated control.