Cocaine Enhances HIV-1 Infectivity in Monocyte Derived Dendritic Cells by Suppressing microRNA-155
Figure 3
miR-155 inhibition enhances HIV-1 replication.
MDDCs (1×106 cells/ml) from normal donors were transfected with anti-miR-155, at a final concentration of 75 nM, followed by infection with HIV-1 (20 ng) and treatment ± cocaine (1 µM). Total RNA was extracted and amplified by RT-PCR for HIV-1 LTR/RU-5 gene, to detect early steps in HIV reverse transcription. Significance is calculated with respect to non targeting HIV-1 control cells. Graphs are represented as % difference from control. Results shown are representative of mean ± SEM of three experiments. p-value≤0.05 were considered to be significant.