A Trio of Viral Proteins Tunes Aphid-Plant Interactions in Arabidopsis thaliana
Figure 7
Disruption of AGO1 function by Fny2b induced antibiosis but not feeding deterrence to aphids.
(A, D) Growth rate of individual aphids feeding on transgenic plants expressing the Fny2b or LS2b proteins and ago1-25 mutant plants, which are compromised in AGO1 activity, a major molecular target of Fny2b, n≥24. (B, E) Electrical penetration graph analysis of the amount of phloem feeding in Fny2b-transgenic plants and ago1-25 mutants over a 12-hour recording period, broken down into hours 1-6, 7-12, and over the entire period (C, F) Results of host-switching experiments where aphids were moved from their initial hosts (“before switch”) to uninfected, wild-type plants (“after switch”) to see if aphids could recover from any effects of the initial host on aphid growth rate, n≥24. Results of Student’s t-tests are indicated by asterisks: *, P<0.05; **, P<0.01; ***, P<0.001. Error bars represent standard error of the mean.