Preconditioning via Angiotensin Type 2 Receptor Activation Improves Therapeutic Efficacy of Bone Marrow Mononuclear Cells for Cardiac Repair
Figure 1
Effect of AT2R Activation on Cardiomyocyte Protection of BMMNCs in vitro.
(A to F) BMMNCs were initially pre-incubated with DMEM, Ang II, CGP42112A, AngII+Valsartan, or AngII+Valsartan+PD123319 for 2 hours at 37°C, respectively. Then preconditioned BMMNCs were co-cultured with NRCMs under hypoxia in serum free medium for 48 hours. Apoptotic NRCMs were detected using TUNEL assay. Bar=100μm; Green represents TUNEL positive cells; Red represents Troponin T (TnT); Blue represents nuclei; and yellow arrowhead represents apoptotic NRCMs. (G) Quantification of apoptotic NRCMs. n=3 for each group; *P < 0.05 versus NRCMs single culture group and BMMNCs group, #P < 0.05 versus BMMNCs+AngII+Valsartan+PD123319 group.