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Traceless Bioresponsive Shielding of Adenovirus Hexon with HPMA Copolymers Maintains Transduction Capacity In Vitro and In Vivo

Figure 5

Positively charged bioresponsive HPMA copolymers mediated robust transduction of liver.

Female BALB/c mice were injected with HPMA copolymer-shielded EGFP-expressing Ad vector particles. 72 h later livers were harvested and processed for fluorimetry (A) and QPCR analysis (B). A: A part of shock frozen liver was homogenized and the relative EGFP expression was analyzed by fluorimetry. B: DNA was isolated from a part of shock frozen liver and was analyzed for its Ad-genome content by QPCR. Abbreviations: cont.: untreated control, HexCys: unshielded AdHexCys, the “+ Polymer-number” indicates a shielding of AdHexCys with the respective HPMA copolymer, neut.: neutral (uncharged), pos.: positively charged, irrev.: irreversible (mal-)shielding, rev.: bioresponsive (PySS-)shielding, n.s.: not significant, p<0.05 (*), p<0.005 (**), significance levels referring to unshielded AdHexCys except when indicated differently.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0082716.g005