Development, Alteration and Real Time Dynamics of Conjunctiva-Associated Lymphoid Tissue
Figure 7
Classification of CALT compartments and according cellular velocities.
A) Schematic drawing of CALT microcompartment with CALT follicle (1), lymphoepithelium (2) and scattered lymphocytes within subepithelial space (3). B) Cells within the lymphoid follicle featured median velocities of 8.0 µm/min (n = 46 cells, 874 individual measurements). Intraepithelial lymphocytes (lymphoepithelium) demonstrated median velocities of 6.7 µm/min (n = 68 cells, 2101 individual measurements) whereas cells within the subepithelial space demonstrated median velocities of 8.4 µm/min (n = 47 cells, 2416 individual measurements). Statistical analysis demonstrated significant differences of the velocities between lymphoepithelium and subepithelial space (*p<0.05). The data derives from 6 individual experiments with 9 time series/separate sets of data. Statistical analysis included One-Way ANOVA (p = 0.02) for testing of normal distribution, followed by Bonferroni Multiple Comparison Test. Significance values below p<0.05 were considered to be significant. Bartlett-Test was used to analyze data variances (p = 0.4). (p>0.05 n.s., p≤0.05 *). C) Intravital two-photon microscopy of CALT and consecutive tracing of individual cells within the follicle (zone 1, compare to 7A). White spheres represent motile cells, blue spheres represent non motile cells with distinct differing autofluorescence properties and dendritic cell-like morphology (compare supplemental Video 3). D) Lymphoid vessel (Ly) and high endothelial venule (HEV) located in close proximity to a CALT follicle demonstrate cellular transmigration. A lymphocyte migrates into a lymphatic vessel (black arrow), whereas another lymphocyte migrates from a high endothelial venule (white arrow).