Development, Alteration and Real Time Dynamics of Conjunctiva-Associated Lymphoid Tissue
Figure 6
A) Fluorescing E. coli bacteria (green) were transported through the lymphoepithelium of CALT and detected close to MHC II positive cells (red) within CALT and around and within lymphatic vessels (asterisk) 60 minutes after application. (Ex vivo confocal microscopy, image stack, steps 1 µm, scale bar 55 µm). B) Intravital two-photon microscopy of CALT lymphoepithelium. An intraepithelial fluorescent microsphere (arrowhead) is approached by a DAPI-labeled lymphocyte (arrow). (Time course in minutes). C+D) Transmission electron microscopy of E. coli particle (arrow) during approach (C) and within (D) a macrophage (asterisk). E) Scanning electron microscopy of lymphoepithelium. Cellular surface villi with typical features of M-cells.