Testing the Reproducibility of Multiple Displacement Amplification on Genomes of Clonal Endosymbiont Populations
Figure 1
Concentration-dependent bias in read coverage of MDA samples obtained from bacterial cells.
The coverage of sequence reads across the B. australis genome is shown for Illumina sequences generated from (A) the unamplified DNA from the previously published B. australis genome project and (B-E) the re-sequencing of the B. australis genome from MDA samples. Four different dilutions of bacterial cells were used as templates for the MDA reaction: (B) "cells2", (C) "cells3", (D) "cells4" and (E) "cells5", as detailed in Table 1. For each plot, the coverage of the unamplified control obtained from the re-sequencing of B. australis in this study is shown in red, and the sample for comparison in blue (two shades, corresponding to two moving average window sizes of 300 bp and 500 bp). The mean coverage of all samples was scaled to be the same as the control sample. (F) The coverage of samples “cells3” and “cells4” were scaled to have the same mean coverage as sample “cells2”, and plotted in the same window to facilitate visualization.