Attenuation of CCl4-Induced Hepatic Fibrosis in Mice by Vaccinating against TGF-β1
Figure 4
Antisera raised by TGF-β1 kinoid vaccination inhibit the TGF-β1-induced activation of pSmad2/3 and the expression of α-SMA, COL1A2, PAI-1 and TIMP-1 in HSC-T6 cells.
HSC-T6 cells in 6-well plates were treated with TGF-β1 (6 ng/mL) and the mouse serum (1:200) for 24 hours. The phosphorylation of Smad2/3 and expression of α-SMA in were determined by Western blotting with the expression of Smad2/3 and β-actin serving as the internal controls (A). The expressions of COL1A2 (B), PAI-1 (C) and TIMP-1 (D) mRNA were evaluated by RT-qPCR. The RT-qPCR results represent the means ± the standard error of the mean of three independent experiments each of which was performed in triplicate qPCR reactions. The error bars indicate SEM.