A Lepidopteran-Specific Gene Family Encoding Valine-Rich Midgut Proteins
Figure 6
Immunodetection of MsVmp1 in PM preparations from anterior and posterior midguts of feeding larvae.
(A) PM proteins were extracted by SDS treatment and separated by SDS-PAGE. Then the proteins were transferred to nitrocellulose and reacted with polyclonal anti-Vmp1 antibodies. Std, standard proteins with molecular masses indicated in kDa. (B) Immunodetection of Vmps using anti-Vmp1 antibodies. The PM preparations from the anterior and posterior parts of the midgut were washed several times with PBS buffer, blocked with bovine serum albumin and stained with the anti-Vmp1 antibodies. Cy3-conjugated anti-guinea pig IgGs were used as secondary antibodies. The PM was transferred to a microscope slide and mounted with Vectashield under a cover slip. The specimens were viewed under a fluorescence microscope using appropriate excitation an emission filters.