Ubiquitin Ligase HUWE1 Regulates Axon Branching through the Wnt/β-Catenin Pathway in a Drosophila Model for Intellectual Disability
Figure 1
HUWE1 overexpression does not affect learning or MB development.
(A) Short term memory (STM) was measured in the courtship conditioning paradigm in UAS-HUWE1VK37/+;UAS-HUWE1VK31/247-Gal4 males kept at 28°C. CI = courtship time / total time; LI = (CInaïve-CItrained) / CInaïve. Error bars represent the SEM. (B-C) Mushroom body analysis (B,B’) Control Canton S10 (CanS10) and UAS-HUWE1VK31 lines were crossed to the 201Y-Gal4,UAS-DenMark,UAS-mCD8-GFP driver line and kept at 25°C. The images are composites of a Z-projection of the confocal sections containing the axon lobes labeled by UAS-mCD8-GFP in green, and a Z-projection of the sections containing the calyx, labeled by DenMark in red. No gross abnormalities were observed in the axon lobes or the calyx. (C,C’) Expression of HUWE1 driven by the 247-Gal4 driver from the double transgenic ;UAS-HUWE1VK37;UAS-HUWE1VK31 line did not affect the morphology of the axon lobes when the flies were raised at 28°C. CanS10 flies were used as controls. The MB lobes were visualized by anti-FasII staining.