Trichostatin A Stabilizes the Expression of Pluripotent Genes in Human Mesenchymal Stem Cells during Ex Vivo Expansion
Figure 5
Influence of TSA on the expression and histone H3 acetylation of pluripotent gene in hMSCs.
Passage (P)1 hMSCs were grown in the presence of TSA (at 6.25 nM) or vehicle DMSO to passage 6. (A) Real-Time PCR analysis of Oct4, TERT, Sox2, Nanog, REX1 and CD133 genes of the cells in P1 and P6 (A, n=3, *P<0.01 when P6-DMSO compared to P1 and to P6-TSA). (B) Acetylation of histone H3 in K9 and K14 in Oct4, Sox2 and TERT genes in DMSO- or TSA-treated hMSCs (*P<0.01 when P6-DMSO compared to P1 and to P6-TSA). The experiments were repeated three times with similar results.