Swing-Out of the β3 Hybrid Domain Is Required for αIIbβ3 Priming and Normal Cytoskeletal Reorganization, but Not Adhesion to Immobilized Fibrinogen
Figure 8
Cells expressing XS-O mutant 321/358 can retract fibrin clots.
Untransduced cells (control 293 cells), cells expressing normal αIIbβ, and cells expressing XS-O mutant 321/358 were incubated with 200 µg/ml fibrinogen and 2 U/ml thrombin at 37°C. Clot retraction was monitored by photography at timed intervals up to 4 hr. The 45 min time point is shown since maximal clot retraction was achieved at this time.