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Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease

Figure 3

Network and evolutionary relationships of Apicystis bombi haplotypes.

(a) Bees in Europe (blue), Argentina (green), Mexico (red), and a commercially produced bumblebee colony in Europe (yellow). Circle sizes are proportional to the numbers of bees infected with the haplotype and the circle colors indicate the proportion of bees with the haplotype that came from each location. The black squares represent unobserved single-nucleotide substitutions. (b) The geographic location in which each haplotype was detected is indicated with colored spots for Europe (blue), Argentina (green), Mexico (red), and a commercially produced bumblebee colony in Europe (yellow). The optimal tree with the sum of branch length = 0.0176 is shown. The percentages of replicate trees in which the associated haplotypes clustered together in the bootstrap test (1000 replicates) are shown next to the branches.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0081475.g003