5-Aminolevulinic Acid Protects against Cisplatin-Induced Nephrotoxicity without Compromising the Anticancer Efficiency of Cisplatin in Rats In Vitro and In Vivo
Figure 3
Tubular cell apoptosis and levels of cleaved caspase3 in renal tissues of ALA treated rats with cisplatin-inducedAKI.
Kidneys were removed 5(8 mg/kg). A, B) Kidneys of cisplatin-treated rats exhibited an elevated number of TUNEL-positive renal tubular cells. (Magnification, X100). C, D) Kidneys of ALA treatment (both post and pre & post) show a very few number of TUNEL-positive renal tubular cells. (Magnification, X100) E) The number of TUNEL positive tubular cells was significantly low in ALA (both post and pre & post) treated rat kidneys in cisplatin-induced AKI. (F) Western blot analysis of cleaved caspase3 were performed in each group rats. (G) Quantitative densitometry was performed for cleaved caspase 3 blots. Data are the mean ± SEM of 6 rats per group. Statistically significant differences (*p<0.05) are indicated.