Immunoproteomic Analysis of Proteins Expressed by Two Related Pathogens, Burkholderia multivorans and Burkholderia cenocepacia, during Human Infection
Figure 1
Representative Coomassie blue stained 2-D gel (pI 3-11) of B. cenocepacia strains, C1394 (A) and BC-7 (B) and the corresponding Western blots probed with pooled patient serum from Bcc colonised patients (C and D) or with serum from patients with no history of Bcc colonisation (E and F).
Each gel was prepared with 120 µg membrane protein preparations extracted from 18 h cultures grown at 37 °C, focused on IEF strips (pH 3 to pH 11) separated on 12% SDS-PAGE gels, , blotted and probed the respective sera and detected with anti-human IgG. The corresponding spots on the gel were excised and identified by MALDI-ToF/MS analysis. The numbers represent the proteins spots referred to in the text. The approximate positions of molecular mass markers (kDa) are indicated.