Glycation Accelerates Fibrillization of the Amyloidogenic W7FW14F Apomyoglobin
Figure 8
Effect of ribosylation on the cytotoxicity of the W7FW17F apomyoglobin aggregates.
NIH-3T3 cells were exposed to aggregates formed in the absence (light grey) and in the presence of D-ribose (dark grey). Aliquots of protein were taken at 0, 5 and 15 days from the onset of aggregation and incubated for 24 h with cells. Data are expressed as average percentage of MTT reduction ±SD relative to cell treated with medium alone or medium plus D-ribose, from triplicate wells from 5 separate experiments (p<0.01). The white bar represents the negative control (cells treated with medium plus 0.25 M D-ribose). Other experimental conditions are described in the Materials and Methods section.