Transdifferentiation of Adipose-Derived Stem Cells into Keratinocyte-Like Cells: Engineering a Stratified Epidermis
Figure 1
Isolation and characterization of ASC.
ASC were isolated and sorted from human fat using fluorescence-activated cell sorting. (a) 92% of the total SVF expressed CD90 and CD105 and were negative for CD31 and CD45. Interestingly these cells (CD90+CD105+ and CD31−CD45−) were non-reactive to CD34 antibody. Cells that expressed CD90+CD105+ and negative for CD31−CD45− were sorted, collected and cultured for further experiments. (b) Sorted ASC exhibited a spindle-like shape (80–100 µm in diameter and ∼200 µm in length) Scale bars indicate 100 µm. (c) ASC were cultured under adipogenic conditions for up to 7 days revealing formation of intracellular microdroplets demonstrated by Oil Red O staining. Scale bar indicating 50 µm. All images in this figure were captured with a Nikon digital sight DS-2MBW camera on a Nikon Eclipse TS100 light-microscope at 20x magnification. (Image representative n = 3 independent experiments).