TLR4-MyD88/Mal-NF-kB Axis Is Involved in Infection of HSV-2 in Human Cervical Epithelial Cells
Figure 5
TLR4 mediates the activation of NF-kB signaling in response to HSV-2.
HCE cells were transiently transfected with vector or iTLR4. Forty-eight hours after transfection, cells were infected with HSV-2 or mock-infected for 0 h, 1 h, 2 h and 4 h respectively. Cell lysates were analyzed by Western blotting with antibodies against p-IkB-α, total IkB-α and actin. Knockdown of TLR4 expression decreased phosphorylated IkB-α. The experiment was repeated three times, the representative images from one experiment are shown.