A Novel Chordoma Xenograft Allows In Vivo Drug Testing and Reveals the Importance of NF-κB Signaling in Chordoma Biology
Figure 1
A) CT scan showing patient’s primary sacral chordoma.
Tumor is indicated by the arrow. B) CT scan showing the patient’s metastatic relapse to the chest wall (indicated by the arrow). C) Photograph of a mouse bearing the xenograft established from a sample of the patient’s primary tumor. D) RT-PCR showing brachyury expression in xenograft. GAPDH is used as a loading control and control for RNA integrity. RNA from the JHC7 cell line (a kind gift from Dr. A Quinones-Hinojosa, Johns Hopkins University [10]) and a sample from an unrelated patient’s chordoma (Prim) were used as positive controls for brachyury expression, and a sample prepared without reverse transcriptase (no RT) served as a negative control. The upper arrow indicates the brachyury amplicon, and the lower arrow indicates the GAPDH control. The identity of the brachyury band was confirmed by direct sequencing of the PCR product.