Role of TRPM7 Channels in Hyperglycemia-Mediated Injury of Vascular Endothelial Cells
Figure 5
Effect of TRPM7 siRNA on MAPK pathway in HG treated HUVECs.
The cells were preincubated with TRPM7 siRNA or control siRNA for 48h, and then stimulated with HG for 72h. (A) Representative immunoblots showing phospho-ERK1/2, phospho-p38MAPK and phospho-JNK protein expression level. (B) The corresponding bar showing the relative optical density of phospho-ERK1/2 protein normalized to total ERK1/2. **p<0.01 vs. control; ##p<0.01 vs. control siRNA. n=5 for immunoblotting (C) The corresponding bar graphs showing the relative optical density of phospho-p38MAPK protein normalized to beta-actin. (D) The corresponding bar graphs showing the relative expression of phospho-JNK protein normalized to beta-actin. n=4.