Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
Figure 8
Menaquinone derivatives lacking an isoprenoid side chain cannot suppress the complex colony development defect in B. subtilis siderophore mutants.
Three μl of a fresh overnight culture were spotted onto the center of spreading plates containing 1.2% agar, spreading plates containing 1.2% agar supplemented with 40 μg/ml of either MK-4, phylloquinone, menadione sodium bisulfite (MBS) or Hydroquinone (HyQ), or spreading plates containing 1.2% agar supplemented with 10 μg/ml of menadione. Photos were taken after 72 hours. Each experiment was carried out in triplicate.