Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
Figure 4
Reconstitution of complex colony development by addition of MK-4.
Three μl of a fresh overnight culture were spotted onto the center of spreading plates containing 1.2% agar (left) and spreading plates containing 1.2% agar supplemented with 40 μg/ml MK-4 (right) and incubated at 37°C in a humid and dark environment. Plates were photographed after 72 hours. The presented results are representative of at least five independent experiments.