Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
Figure 3
The menG mutant is defective in complex colony development and growth.
A. Cells of wild-type (left) and menG (right) strains were spotted on spreading plates containing 1.2% agar and incubated at 37°C in a humid and dark environment. Plates were photographed after 72 hours. The presented results are representative of five independent experiments. B. Growth curves were performed in spreading medium or in spreading medium supplemented with 40 μg/ml of either MK-4 or phylloquinone. The presented results are representative of three independent experiments.