Cold-Inducible RNA-Binding Protein Is an Important Mediator of Alcohol-Induced Brain Inflammation
Figure 1
Alcohol increased brain CIRP level in a time dependent manner.
WT mice were infused intravenously with alcohol as described in Materials and Methods. Whole brain tissue from WT mice was collected at 5, 10 and 15(A) CIRP mRNA expression was determined by real time RT-PCR analysis and expression levels were normalized to β-actin. (B) Tissue lysates were collected and analyzed for CIRP by Western blotting. Blots were scanned and quantified by densitometry. Band intensity of CIRP was normalized to the corresponding band intensity of β-actin. The ratio of the control group is designated as 1 for comparison. Data presented as means ± SE (n = 3–4/group) and compared by one-way ANOVA and SNK method; *p<0.05 vs. Sham.