Roscovitine-induced Apoptosis in Neutrophils and Neutrophil Progenitors Is Regulated by the Bcl-2-Family Members Bim, Puma, Noxa and Mcl-1
Figure 5
Role of caspases and BH3-only proteins in apoptosis in neutrophil progenitor cells.
(A) PI-staining of wt progenitor cells treated with roscovitine (25 µM) alone or in the presence of the caspase-inhibitor Q-VD-OPh (50 µM) for 12 h. (B) PI staining of wt, Bim-/-, Noxa-/-, Bim/Noxa-/-, Bim/Puma-/- and Bcl-2-transgenic progenitors treated with roscovitine (25 µM) for the indicated times. Bar diagram represents mean/SEM of three independent experiments. Statistical significance is calculated using the Student’s t-test (ns, non-significant; *, p<0.05; ** p< 0.01; *** p<0.001).