Avian Influenza Virus H3 Hemagglutinin May Enable High Fitness of Novel Human Virus Reassortants
Figure 4
Growth curves of MallGer05 HA reassortants.
The gene segment composition of the each reassortant is given next to each chart: Hk68 genes (black) and MallGer05 genes (red). The presence of diverse additional MallGer05 segments is indicated by the abbreviation ‘div’. From supernatants of A549 cells infected with a MOI of 10−3, viral titers were determined by plaque assays on MDCK cells. The growth curves of the parental viruses Hk68 (black filled circles), Hk68-Ela (black empty circles) and MallGer05 (red filled circles) in each chart represent identical data and were drawn in for comparison. Growth curves were determined by plaque titrations of duplicate or quadruplicate infected cell cultures (in the latter case indicated by a diamond in the chart legend). Reassortants reaching titers at 48 h not less than one magnitude below Hk68 are highlighted by grey rectangles.