Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase
Figure 6
Comparison of cytosine and 5-methylcytosine deamination by M.SssI.
Unmethylated and in vivo M.SssI-specifically methylated pUP41 plasmid DNA was incubated with or without M.SssI(WT) and 5’-amino-5’-deoxyadenosine as indicated using conditions described in the legend of Figure 1. Frequency of reversion to KnR phenotype was determined for unmethylated pUP41 in ER2357 ung (empty bars), whereas reversion frequency of methylated pUP41 was determined in DH10B ung+ host (filled bars). Error bars represent standard error of the mean of at least three independent experiments (p<0.01).