A ΔclpB Mutant of Francisella tularensis Subspecies holarctica Strain, FSC200, Is a More Effective Live Vaccine than F. tularensis LVS in a Mouse Respiratory Challenge Model of Tularemia
Figure 6
Immunoreactivity of sera from BALB/c mice after vaccination with FSC200ΔclpB or LVS.
Mice were bled 28 days after immunization with 105 CFU of FSC200ΔclpB or LVS. Each pool contained sera from five mice. Western blots were developed and scanned, and immunoreactive areas recorded as relative immunoreactivity, measured by densitometry. Shown are the top ranked immunodominant proteins. The identity of each immunoreactive area was determined by alignment with an equivalent protein stained gel, and immunoreactive spots identified by nLC-MSMS of their tryptic digests.