Fhit Delocalizes Annexin A4 from Plasma Membrane to Cytosol and Sensitizes Lung Cancer Cells to Paclitaxel
Figure 2
Fhit overexpression blocks Annexin 4 translocation from cytosol to plasma membrane.
A-C. A549 and Calu-2 lung cancer cells were infected with AdGFP or AdFHIT at MOI50; 48 h later, cells were mock-treated or treated with paclitaxel (800 nM) for an additional 24 h. Proteins from cytosolic and membrane fractions were separated on a polyacrylamide gel, transferred to nitrocellulose filter, and probed with Annexin 4 antibody. Gapdh and E-cadherin were used to normalize protein loading of cytosolic and plasma membrane proteins, respectively. B-D. A549 and Calu-2 lung cancer cells were infected with AdGFP or AdFHIT-wild-type; 48 h later, cells were treated with paclitaxel (800 nM) for an additional 24 h. Proteins from cytosolic and membrane fractions were separated on a polyacrylamide gel, transferred to nitrocellulose filter, and probed with Annexin 1 and MDR (multi-drug resistance protein) antibodies. Gapdh and E-cadherin were used to normalize protein loading of cytosolic and plasma membrane proteins, respectively.