Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence
Figure 9
Changes in expression of target genes following exosomes transfection.
Quantitative real-time PCR were carried out using gene-selective primers. miRNA target genes were selected using seed match and their involvement in important pathways. To elucidate the exosome mediated uptake of extra-cellular miRNAs can alter the abundance of target mRNAs we used the same exosome co-cultured granulosa cells which was used for miRNA abundance study. The histogram in this figure indicated that significantly lower expression of target mRNAs except VEGFA, suggesting possible involvement of transcriptional regulation of circulating miRNA. Different superscript letters (a,b) denote a significant difference between groups, such that groups not sharing a similar letter are significantly different from each other (P<0.05). The data is presented as means ± SD of three biological replicates.